P5F1B Antibody

ProSci
Product Code: PSI-55-446
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-446-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Putative POU domain, class 5, transcription factor 1B, Oct4-pg1, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like, POU5F1B
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development.
Background References:
  • Kastler, S., et al. Prostate 70(6):666-674(2010)
  • White, K.L., et al. Twin Res Hum Genet 13(1):43-56(2010)
  • Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)
  • Gudmundsson, J., et al. Nat. Genet. 41(10):1122-1126(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig
Immunogen:
This P5F1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the C-terminal region of human P5F1B.
NCBI Gene ID #:
5462
NCBI Official Name:
Putative POU domain, class 5, transcription factor 1B
NCBI Official Symbol:
POU5F1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
Q06416
Protein GI Number:
357528826
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q06416
User NOte:
Optimal dilutions for each application to be determined by the researcher.