TDG Antibody
| Code | Size | Price |
|---|
| PSI-62-366-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase, hTDG, TDG
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
The protein TDG belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine.
Background References:
- Kim,E.J., Biochem. Biophys. Res. Commun. 377 (3), 838-842 (2008)
- Neddermann,P., J. Biol. Chem. 271 (22), 12767-12774 (1996)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TDG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the C-terminal region of human TDG.
NCBI Gene ID #:
6996
NCBI Official Name:
G/T mismatch-specific thymine DNA glycosylase
NCBI Official Symbol:
TDG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
Q13569
Protein GI Number:
46397791
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q13569
User NOte:
Optimal dilutions for each application to be determined by the researcher.