RAD54 Antibody

ProSci
Product Code: PSI-56-150
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-150-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
DNA repair and recombination protein RAD54-like, 364-, RAD54 homolog, hHR54, hRAD54, RAD54L, RAD54A
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.
Background References:
  • Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010)
  • Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
  • Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :
  • Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse
Immunogen:
This RAD54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 634-663 amino acids from the C-terminal region of human RAD54.
NCBI Gene ID #:
8438
NCBI Official Name:
DNA repair and recombination protein RAD54-like
NCBI Official Symbol:
RAD54L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
84 kDa
Protein Accession #:
Q92698
Protein GI Number:
51316508
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q92698
User NOte:
Optimal dilutions for each application to be determined by the researcher.