ELF4 Antibody

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ProSci
Product Code: PSI-62-712
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-712-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).
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Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
ETS-related transcription factor Elf-4, E74-like factor 4, Myeloid Elf-1-like factor, ELF4 {ECO:0000312|EMBL:CAI428821}
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
ELF4 is transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. The protein acts synergistically with RUNX1 to transactivate the IL3 promoter. Also transactivates the PRF1 promoter in natural killer (NK) cells. This protein plays a role in the development and function of NK and NK T-cells and in innate immunity.
Background References:
  • Miyazaki Y., Sun X.Oncogene 13:1721-1729(1996)
  • Aryee D.N.T., Petermann R.Gene 210:71-78(1998)
  • Mao S., Frank R.C., Zhang J.Mol. Cell. Biol. 19:3635-3644(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ELF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-237 amino acids from the Central region of human ELF4.
NCBI Gene ID #:
2000
NCBI Official Name:
ETS-related transcription factor Elf-4
NCBI Official Symbol:
ELF4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
71 kDa
Protein Accession #:
Q99607
Protein GI Number:
68052244
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q99607
User NOte:
Optimal dilutions for each application to be determined by the researcher.