SP6 Antibody

ProSci
Product Code: PSI-56-797
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-797-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Transcription factor Sp6, Krueppel-like factor 14, SP6, KLF14
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes (Scohy et al., 2000 [PubMed 11087666]).
Background References:
  • Nakamura, T., et al. J. Biol. Chem. 279(1):626-634(2004)
  • Scohy, S., et al. Genomics 70(1):93-101(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This SP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the Central region of human SP6.
NCBI Gene ID #:
80320
NCBI Official Name:
Transcription factor Sp6
NCBI Official Symbol:
SP6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
Q3SY56
Protein GI Number:
82582258
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q3SY56
User NOte:
Optimal dilutions for each application to be determined by the researcher.