FOXK1 Antibody

ProSci
Product Code: PSI-64-162
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-162-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western Blot at 1:500 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 2
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:500 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Forkhead box protein K1, Myocyte nuclear factor, MNF, FOXK1 {ECO:0000303|PubMed:15202027, ECO:0000303|PubMed:15289879}
Application Note:
For WB starting dilution is: 1:500

For FACS starting dilution is: 1:10~50
Background:
FOXK1 is a transcriptional activator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. It plays a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.
Background References:
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Tsai, K.L., et al. J. Biol. Chem. 281(25):17400-17409(2006)
  • Huang, J.T., et al. Int. J. Oncol. 25(3):751-757(2004)
  • Katoh, M., et al. Int. J. Mol. Med. 14(1):127-132(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This FOXK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-710 amino acids from the C-terminal region of human FOXK1.
NCBI Gene ID #:
221937
NCBI Official Name:
Forkhead box protein K1
NCBI Official Symbol:
FOXK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
P85037
Protein GI Number:
118572324
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P85037
User NOte:
Optimal dilutions for each application to be determined by the researcher.