RFC3 Antibody

ProSci
Product Code: PSI-61-558
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-558-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-RFC3 Antibody in 293 cell line lysates (35ug/lane)
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Flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of anti-RFC3 Antibody in 293 cell line lysates (35ug/lane)
Flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Replication factor C subunit 3, Activator 1 38 kDa subunit, A1 38 kDa subunit, Activator 1 subunit 3, Replication factor C 38 kDa subunit, RF-C 38 kDa subunit, RFC38, RFC3
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa.
Background References:
  • Rauen M., Burtelow M.A.J. Biol. Chem. 275:29767-29771(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This RFC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-150 amino acids from the Central region of human RFC3.
NCBI Gene ID #:
5983
NCBI Official Name:
Replication factor C subunit 3
NCBI Official Symbol:
RFC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
P40938
Protein GI Number:
3915601
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P40938
User NOte:
Optimal dilutions for each application to be determined by the researcher.