FBXO3 Antibody

ProSci
Product Code: PSI-63-831
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-831-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of FBXO3 Antibody in HepG2 cell line lysates (35ug/lane)
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Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of FBXO3 Antibody in HepG2 cell line lysates (35ug/lane)
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
F-box only protein 3, FBXO3, FBX3
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
FBXO3 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs.
Background References:
  • Shima,Y., et.al., Mol. Cell. Biol. 28 (23), 7126-7138 (2008)
  • Ilyin,G.P., et.al., Genomics 67 (1), 40-47 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This FBXO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-422 amino acids from the C-terminal region of human FBXO3.
NCBI Gene ID #:
26273
NCBI Official Name:
F-box only protein 3
NCBI Official Symbol:
FBXO3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
Q9UK99
Protein GI Number:
145559474
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UK99
User NOte:
Optimal dilutions for each application to be determined by the researcher.