CHST2 Antibody

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ProSci
Product Code: PSI-55-356
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-356-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Carbohydrate sulfotransferase 2, 282-, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2, GST-2, N-acetylglucosamine 6-O-sulfotransferase 1, GlcNAc6ST-1, Gn6ST-1, CHST2, GN6ST
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).
Background References:
  • Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
  • Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
  • Desko, M.M., et al. Glycobiology 19(10):1068-1077(2009)
  • Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This CHST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-335 amino acids from the Central region of human CHST2.
NCBI Gene ID #:
9435
NCBI Official Name:
Carbohydrate sulfotransferase 2
NCBI Official Symbol:
CHST2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
Q9Y4C5
Protein GI Number:
61212252
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9Y4C5
User NOte:
Optimal dilutions for each application to be determined by the researcher.