PGAP2 Antibody

ProSci
Product Code: PSI-62-024
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-024-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (15ug/lane).
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in HL-60 cell line lysates (15ug/lane).
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Post-GPI attachment to proteins factor 2, FGF receptor-activating protein 1, PGAP2, FRAG1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
PGAP2 is involved in the lipid remodeling steps of GPI-anchor maturation. Required for stable expression of GPI-anchored proteins at the cell surface (By similarity).
Background References:
  • Tashima, Y., et al. Mol. Biol. Cell 17(3):1410-1420(2006)
  • Lorenzi, M.V., et al. Genomics 62(1):59-66(1999)
  • Robertson, N.G., et al. Genomics 23(1):42-50(1994)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Hamster, Mouse, Rat
Immunogen:
This PGAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the C-terminal region of human PGAP2.
NCBI Gene ID #:
27315
NCBI Official Name:
Post-GPI attachment to proteins factor 2
NCBI Official Symbol:
PGAP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
Q9UHJ9
Protein GI Number:
544584767
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UHJ9
User NOte:
Optimal dilutions for each application to be determined by the researcher.