FBXL14 Antibody

ProSci
Product Code: PSI-61-838
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-838-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane). ?This demonstrates the FBXL14 antibody detected the FBXL14 protein (arrow).
2 / 2
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in MDA-MB435 cell line lysates (35ug/lane). ?This demonstrates the FBXL14 antibody detected the FBXL14 protein (arrow).
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
F-box/LRR-repeat protein 14, F-box and leucine-rich repeat protein 14, FBXL14, FBL14
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
FBXL14 is members of the F-box protein family, such as FBXL14, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.
Background References:
  • Vinas-Castells, R., et al. J. Biol. Chem. 285(6):3794-3805(2010)
  • Jin, J., et al. Genes Dev. 18(21):2573-2580(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FBXL14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-407 amino acids from the C-terminal region of human FBXL14.
NCBI Gene ID #:
144699
NCBI Official Name:
F-box/LRR-repeat protein 14
NCBI Official Symbol:
FBXL14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
Q8N1E6
Protein GI Number:
48428083
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8N1E6
User NOte:
Optimal dilutions for each application to be determined by the researcher.