RPL14 Antibody

Contact us
ProSci
Product Code: PSI-61-163
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-163-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 2
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: 293T/17 whole cell lysate Lane 5: U-2OS whole cell lysate Lane 6: A431 whole cell lysate Lysates/proteins at 20 ug per lane.
❮❯

Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: 293T/17 whole cell lysate Lane 5: U-2OS whole cell lysate Lane 6: A431 whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
60S ribosomal protein L14, CAG-ISL 7, RPL14
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background References:
  • Aoki M.,et al.Diabetes 45:157-164(1996).
  • Tanaka M.,et al.Biochem. Biophys. Res. Commun. 243:531-537(1998).
  • Yoshihama M.,et al.Genome Res. 12:379-390(2002).
  • Lin L.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RPL14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 117-147 amino acids from the Central region of human RPL14.
NCBI Gene ID #:
9045
NCBI Official Name:
60S ribosomal protein L14
NCBI Official Symbol:
RPL14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
P50914
Protein GI Number:
212276521
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P50914
User NOte:
Optimal dilutions for each application to be determined by the researcher.