RPL4 Antibody

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ProSci
Product Code: PSI-61-256
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-256-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: NCI-H929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: NCI-H929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

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Further Information

Additional Names:
60S ribosomal protein L4, 60S ribosomal protein L1, RPL4, RPL1
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background References:
  • Bagni C.,et al.Biochim. Biophys. Acta 1216:475-478(1993).
  • Bagni C.,et al.Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
  • Kato S.,et al.Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
  • Yoshihama M.,et al.Genome Res. 12:379-390(2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This RPL4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-149 amino acids from human RPL4.
NCBI Gene ID #:
6124
NCBI Official Name:
60S ribosomal protein L4
NCBI Official Symbol:
RPL4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
P36578
Protein GI Number:
22002063
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P36578
User NOte:
Optimal dilutions for each application to be determined by the researcher.