ATP8A2 Antibody

ProSci
Product Code: PSI-55-517
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-517-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in K562 cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Phospholipid-transporting ATPase IB, ATPase class I type 8A member 2, ML-1, P4-ATPase flippase complex alpha subunit ATP8A2, ATP8A2, ATPIB
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Coleman, J.A., et al. J. Biol. Chem. 284(47):32670-32679(2009)
  • Dunham, A., et al. Nature 428(6982):522-528(2004)
  • Halleck, M.S., et al. Physiol. Genomics 1(3):139-150(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ATP8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from the N-terminal region of human ATP8A2.
NCBI Gene ID #:
51761
NCBI Official Name:
Phospholipid-transporting ATPase IB
NCBI Official Symbol:
ATP8A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
129 kDa
Protein Accession #:
Q9NTI2
Protein GI Number:
30316390
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9NTI2
User NOte:
Optimal dilutions for each application to be determined by the researcher.