ZNF207 Antibody

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ProSci
Product Code: PSI-57-696
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-696-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HepG2 cell line lysates (35ug/lane).
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Fluorescent confocal image of A549 cell stained with ZNF207 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF207 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZNF207 immunoreactivity is localized to Nucleus significantly.
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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Fluorescent confocal image of A549 cell stained with ZNF207 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF207 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZNF207 immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
BUB3-interacting and GLEBS motif-containing protein ZNF207, BuGZ, Zinc finger protein 207, ZNF207, BUGZ
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
ZNF207 is a new candidate transcription factor.
Background References:
  • Andersen, J.S., et al. Nature 433(7021):77-83(2005)
  • Pahl, P.M., et al. Genomics 53(3):410-412(1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ZNF207 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-116 amino acids from the N-terminal region of human ZNF207.
NCBI Gene ID #:
7756
NCBI Official Name:
BUB3-interacting and GLEBS motif-containing protein ZNF207
NCBI Official Symbol:
ZNF207
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
O43670
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O43670
User NOte:
Optimal dilutions for each application to be determined by the researcher.