ARGFX Antibody

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ProSci
Product Code: PSI-59-376
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-59-376-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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Fluorescent confocal image of HepG2 cell stained with ARGFX Antibody .HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGFX primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ARGFX immunoreactivity is localized to Nucleus significantly.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Fluorescent confocal image of HepG2 cell stained with ARGFX Antibody .HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGFX primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ARGFX immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
Arginine-fifty homeobox, ARGFX
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
Homeobox genes encode DNA-binding proteins, many of which are thought to be involved in early embryonic development. Homeobox genes encode a DNA-binding domain of 60 to 63 amino acids referred to as the homeodomain. This gene is a member of the ARGFX homeobox gene family.
Background References:
  • Booth, H.A., et al. Gene 387 (1-2), 7-14 (2007) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ARGFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human ARGFX.
NCBI Gene ID #:
503582
NCBI Official Name:
Arginine-fifty homeobox
NCBI Official Symbol:
ARGFX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
A6NJG6
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
A6NJG6
User NOte:
Optimal dilutions for each application to be determined by the researcher.