FOSL2 Antibody

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ProSci
Product Code: PSI-58-520
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-520-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in U251 cell line lysates (35ug/lane).
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Fluorescent confocal image of A549 cell stained with FOSL2 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOSL2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOSL2 immunoreactivity is localized to Nucleus significantly.
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Western blot analysis in U251 cell line lysates (35ug/lane).
Fluorescent confocal image of A549 cell stained with FOSL2 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOSL2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOSL2 immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
Fos-related antigen 2, FRA-2, FOSL2, FRA2
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.
Background References:
  • Bozec, A., et al. J. Cell Biol. 190(6):1093-1106(2010)
  • Bolat, I., et al. Folia Histochem. Cytobiol. 48(2):197-201(2010)
  • Reich, N., et al. Arthritis Rheum. 62(1):280-290(2010)
  • Maurer, B., et al. Circulation 120(23):2367-2376(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FOSL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-248 amino acids from the C-terminal region of human FOSL2.
NCBI Gene ID #:
2355
NCBI Official Name:
Fos-related antigen 2
NCBI Official Symbol:
FOSL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
P15408
Protein GI Number:
120487
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P15408
User NOte:
Optimal dilutions for each application to be determined by the researcher.