HOXA10 Antibody

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ProSci
Product Code: PSI-55-119
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-119-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from T47D cell line, using HOXA10 Antibody at 1:1000.
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Confocal immunofluorescent analysis of HOXA10 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of lysate from T47D cell line, using HOXA10 Antibody at 1:1000.
Confocal immunofluorescent analysis of HOXA10 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Homeobox protein Hox-A10, Homeobox protein Hox-18, Homeobox protein Hox-1H, PL, HOXA10, HOX1H
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor that may regulate gene expression, morphogenesis, and differentiation. More specifically, it may function in fertility, embryo viability, and regulation of hematopoietic lineage commitment. Alternatively spliced transcript variants encoding different isoforms have been described.
Background References:
  • Du, H., et al. Biol. Reprod. 83(2):205-211(2010)
  • Cheng, W., et al. J. Cancer Res. Clin. Oncol. 136(8):1221-1227(2010)
  • Liatsikos, S.A., et al. Reprod. Biomed. Online 21(1):126-132(2010)
  • Godbole, G., et al. J. Reprod. Immunol. 85(2):130-139(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HOXA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-271 amino acids from the Central region of human HOXA10.
NCBI Gene ID #:
3206
NCBI Official Name:
Homeobox protein Hox-A10
NCBI Official Symbol:
HOXA10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
P31260
Protein GI Number:
294862509
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P31260
User NOte:
Optimal dilutions for each application to be determined by the researcher.