TSC22D2 Antibody

Contact us
ProSci
Product Code: PSI-58-548
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-548-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in HepG2 cell line lysates (35ug/lane).
2 / 2
Fluorescent confocal image of A431 cell stained with TSC22D2 Antibody (N-term).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC22D2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TSC22D2 immunoreactivity is localized to Cytoplasm significantly.
❮❯

Western blot analysis in HepG2 cell line lysates (35ug/lane).
Fluorescent confocal image of A431 cell stained with TSC22D2 Antibody (N-term).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC22D2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TSC22D2 immunoreactivity is localized to Cytoplasm significantly.

Documents

Further Information

Additional Names:
TSC22 domain family protein 2, TSC22-related-inducible leucine zipper protein 4, TSC22D2, KIAA0669, TILZ4
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
TSC22D2 belongs to the TSC-22/Dip/Bun family and the function remains unknown.
Background References:
  • Gluderer, S., et al. J. Biol.(1),(2010) :
  • Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)
  • Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TSC22D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-62 amino acids from the N-terminal region of human TSC22D2.
NCBI Gene ID #:
9819
NCBI Official Name:
TSC22 domain family protein 2
NCBI Official Symbol:
TSC22D2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
79 kDa
Protein Accession #:
O75157
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O75157
User NOte:
Optimal dilutions for each application to be determined by the researcher.