HOXC10 Antibody

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ProSci
Product Code: PSI-59-289
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-59-289-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Fluorescent confocal image of MCF-7 cell stained with HOXC10 Antibody .MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXC10 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HOXC10 immunoreactivity is localized to Nucleus significantly.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Fluorescent confocal image of MCF-7 cell stained with HOXC10 Antibody .MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXC10 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HOXC10 immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
Homeobox protein Hox-C10, Homeobox protein Hox-3I, HOXC10, HOX3I
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The protein level is controlled during cell differentiation and proliferation, which may indicate this protein has a role in origin activation.
Background References:
  • Hwang, J.H., et al. Cloning Stem Cells 11(2):269-279(2009)
  • Zhai, Y., et al. Cancer Res. 67(21):10163-10172(2007)
  • Gabellini, D., et al. EMBO J. 22(14):3715-3724(2003)
  • Kosaki, K., et al. Teratology 65(2):50-62(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HOXC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-179 amino acids from the Central region of human HOXC10.
NCBI Gene ID #:
3226
NCBI Official Name:
Homeobox protein Hox-C10
NCBI Official Symbol:
HOXC10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
Q9NYD6
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9NYD6
User NOte:
Optimal dilutions for each application to be determined by the researcher.