NCL Antibody

Contact us
ProSci
Product Code: PSI-57-876
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-876-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in human placenta tissue lysates (35ug/lane).
2 / 2
Fluorescent confocal image of U251 cell stained wit.U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to Nucleus significantly.
❮❯

Western blot analysis in human placenta tissue lysates (35ug/lane).
Fluorescent confocal image of U251 cell stained wit.U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
Nucleolin, Protein C23, NCL
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Background References:
  • Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010)
  • Tulchin, N., et al. Am. J. Pathol. 176(3):1203-1214(2010)
  • Strang, B.L., et al. J. Virol. 84(4):1771-1784(2010)
  • Bertrand, L., et al. J. Virol. 84(1):109-118(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human NCL.
NCBI Gene ID #:
4691
NCBI Official Name:
Nucleolin
NCBI Official Symbol:
NCL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
P19338
Protein GI Number:
90110781
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P19338
User NOte:
Optimal dilutions for each application to be determined by the researcher.