HNRNPD Antibody

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ProSci
Product Code: PSI-58-256
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-256-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in 293T,A431,NCI-H292,Raji cell line lysates (35ug/lane).
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293T,A431,NCI-H292,Raji cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Heterogeneous nuclear ribonucleoprotein D0, hnRNP D0, AU-rich element RNA-binding protein 1, HNRNPD, AUF1, HNRPD
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
Background References:
  • Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010)
  • Zhai, B., et al. J. Biol. Chem. 285(31):23568-23580(2010)
  • Vazquez-Chantada, M., et al. Gastroenterology 138(5):1943-1953(2010)
  • Trojanowicz, B., et al. Endocr. Relat. Cancer 16(3):857-871(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This HNRNPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the N-terminal region of human HNRNPD.
NCBI Gene ID #:
3184
NCBI Official Name:
Heterogeneous nuclear ribonucleoprotein D0
NCBI Official Symbol:
HNRNPD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
Q14103
Protein GI Number:
13124489
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q14103
User NOte:
Optimal dilutions for each application to be determined by the researcher.