ZNF81 Antibody

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ProSci
Product Code: PSI-58-553
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-553-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Fluorescent confocal image of U251 cell stained with ZNF81 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF81 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ZNF81 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.
2 / 2
Western blot analysis in 293 cell line lysates (35ug/lane).
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Fluorescent confocal image of U251 cell stained with ZNF81 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF81 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ZNF81 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.
Western blot analysis in 293 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Zinc finger protein 81, HFZ20, ZNF81
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
This gene encodes a protein that likely functions as a transcription factor. The protein contains an N-terminal KRAB domain and several C2H2-type zinc finger motifs. Mutations in this gene cause mental retardation X-linked type 45 (MRX45). [provided by RefSeq].
Background References:
  • Kleefstra, T., et al. J. Med. Genet. 41(5):394-399(2004)
  • Hamel, B.C., et al. Am. J. Med. Genet. 85(3):290-304(1999)
  • Knight, J.C., et al. Genomics 21(1):180-187(1994)
  • Marino, M., et al. Mamm. Genome 4(5):252-257(1993)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ZNF81 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-197 amino acids from the N-terminal region of human ZNF81.
NCBI Gene ID #:
347344
NCBI Official Name:
Zinc finger protein 81
NCBI Official Symbol:
ZNF81
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
76 kDa
Protein Accession #:
P51508
Protein GI Number:
55977803
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P51508
User NOte:
Optimal dilutions for each application to be determined by the researcher.