NFIC Antibody

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ProSci
Product Code: PSI-58-230
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-230-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.
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Fluorescent confocal image of Hela cell stained with NFIC Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Fluorescent confocal image of Hela cell stained with NFIC Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.

Documents

Further Information

Additional Names:
Nuclear factor 1 C-type, NF1-C, Nuclear factor 1/C, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/C, NF-I/C, NFI-C, TGGCA-binding protein, NFIC, NFI
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
NFIC recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Nilsson, J., et al. Cancer Res. 70(5):2020-2029(2010)
  • Udelhoven, M., et al. J. Mol. Endocrinol. 44(2):99-113(2010)
  • Plasari, G., et al. Mol. Cell. Biol. 29(22):6006-6017(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NFIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-208 amino acids from the Central region of human NFIC.
NCBI Gene ID #:
4782
NCBI Official Name:
Nuclear factor 1 C-type
NCBI Official Symbol:
NFIC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
P08651
Protein GI Number:
14195672
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P08651
User NOte:
Optimal dilutions for each application to be determined by the researcher.