NR2C2 Antibody

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ProSci
Product Code: PSI-60-472
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-472-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Fluorescent confocal image of Hela cell stained with NR2C2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with NR2C2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4, NR2C2, TAK1, TR4
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
Members of the nuclear hormone receptor family, such as NR2C2, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al., 1996 [PubMed 8661150]).
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Srivastava, R., et al. J. Biol. Chem. 285(15):11100-11105(2010)
  • Ear, T., et al. J. Immunol. 184(7):3897-3906(2010)
  • Suzuki, S., et al. J. Biol. Chem. 285(7):4441-4446(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This NR2C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-466 amino acids from the C-terminal region of human NR2C2.
NCBI Gene ID #:
7182
NCBI Official Name:
Nuclear receptor subfamily 2 group C member 2
NCBI Official Symbol:
NR2C2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
P49116
Protein GI Number:
1351190
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P49116
User NOte:
Optimal dilutions for each application to be determined by the researcher.