FOXN3 Antibody

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ProSci
Product Code: PSI-60-414
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-414-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Fluorescent image of Hela cell stained with FOXN3 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).FOXN3 immunoreactivity is localized to Nucleus significantly.
3 / 3
❮❯

Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Fluorescent image of Hela cell stained with FOXN3 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).FOXN3 immunoreactivity is localized to Nucleus significantly.

Documents

Further Information

Additional Names:
Forkhead box protein N3, Checkpoint suppressor 1, FOXN3, C14orf116, CHES1
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in distinct isoforms. [provided by RefSeq].
Background References:
  • Samaan, G., et al. Biochem. Biophys. Res. Commun. 400(1):60-65(2010)
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
  • Markowski, J., et al. Eur Arch Otorhinolaryngol 266(10):1501-1507(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FOXN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human FOXN3.
NCBI Gene ID #:
1112
NCBI Official Name:
Forkhead box protein N3
NCBI Official Symbol:
FOXN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
O00409
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O00409
User NOte:
Optimal dilutions for each application to be determined by the researcher.