TCF25 Antibody

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ProSci
Product Code: PSI-59-087
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-59-087-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Fluorescent image of A549 cell stained with TCF25 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TCF25 immunoreactivity is localized to Cytoplasm significantly.
2 / 2
Western blot analysis in CEM cell line lysates (35ug/lane).
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Fluorescent image of A549 cell stained with TCF25 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TCF25 immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis in CEM cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Transcription factor 25, TCF-25, Nuclear localized protein 1, TCF25, KIAA1049, NULP1
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development (Steen and Lindholm, 2008 [PubMed 18068114]).[supplied by OMIM].
Background References:
  • Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
  • Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
  • Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
  • Steen, H., et al. Biochem. Biophys. Res. Commun. 366(2):432-437(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25.
NCBI Gene ID #:
22980
NCBI Official Name:
Transcription factor 25
NCBI Official Symbol:
TCF25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
Q9BQ70
Protein GI Number:
23396596
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BQ70
User NOte:
Optimal dilutions for each application to be determined by the researcher.