ATF7 Antibody

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ProSci
Product Code: PSI-63-666
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-666-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in huamn placenta tissue lysates (35ug/lane).
2 / 3
Fluorescent confocal image of Hela cell stained with ATF7 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATF7 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ATF7 immunoreactivity is localized to Nucleus and Cytoplasm significantly.
3 / 3
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Western blot analysis in huamn placenta tissue lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with ATF7 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATF7 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ATF7 immunoreactivity is localized to Nucleus and Cytoplasm significantly.

Documents

Further Information

Additional Names:
Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7, Activating transcription factor 7, Transcription factor ATF-A, ATF7, ATFA
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The cyclic AMP-dependent transcription factor ATF7 is a member of the bZIP family of proteins and is known to bind to the cAMP response element (CRE) as a homodimer.
Background References:
  • Hamard,P.J.,et.al., Oncogene 24 (21), 3472-3483 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ATF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human ATF7.
NCBI Gene ID #:
11016
NCBI Official Name:
Cyclic AMP-dependent transcription factor ATF-7
NCBI Official Symbol:
ATF7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
P17544
Protein GI Number:
12643393
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P17544
User NOte:
Optimal dilutions for each application to be determined by the researcher.