POLI Antibody
Code | Size | Price |
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PSI-57-789-400ul | 400ul | £626.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
DNA polymerase iota, Eta2, RAD30 homolog B, POLI, RAD30B
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Background References:
- Arora, M., et al. Leukemia 24(8):1470-1475(2010)
- Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)
- Kazakov, A.A., et al. Biochemistry Mosc. 75(7):905-911(2010)
- Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This POLI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 673-701 amino acids from the C-terminal region of human POLI.
NCBI Gene ID #:
11201
NCBI Official Name:
DNA polymerase iota
NCBI Official Symbol:
POLI
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
Q9UNA4
Protein GI Number:
327478565
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UNA4
User NOte:
Optimal dilutions for each application to be determined by the researcher.