HRC Antibody

ProSci
Product Code: PSI-56-609
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-609-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
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HRC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
HRC Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Sarcoplasmic reticulum histidine-rich calcium-binding protein, HRC, HCP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a luminal sarcoplasmic reticulum protein identified by its ability to bind low-density lipoprotein with high affinity. The protein interacts with the cytoplasmic domain of triadin, the main transmembrane protein of the junctional sarcoplasmic reticulum (SR) of skeletal muscle. The protein functions in the regulation of releasable calcium into the SR.
Background References:
  • Arvanitis, D.A., et al. Eur. Heart J. 29(20):2514-2525(2008)
  • Arvanitis, D.A., et al. Am. J. Physiol. Heart Circ. Physiol. 293 (3), H1581-H1589 (2007) :
  • Fan, G.C., et al. Am. J. Physiol. Heart Circ. Physiol. 287 (4), H1705-H1711 (2004) :
  • Anderson, J.P., et al. Mol. Cell. Biol. 24(9):3757-3768(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human HRC.
NCBI Gene ID #:
3270
NCBI Official Name:
Sarcoplasmic reticulum histidine-rich calcium-binding protein
NCBI Official Symbol:
HRC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
P23327
Protein GI Number:
134873
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P23327
User NOte:
Optimal dilutions for each application to be determined by the researcher.