CARS2 Antibody

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ProSci
Product Code: PSI-63-238
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-238-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:1000 dilution + NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Probable cysteine--tRNA ligase, mitochondrial, Cysteinyl-tRNA synthetase, CysRS, CARS2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
CARS is a class 2 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. It catalyzes the chemical reaction: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Background References:
  • Bonnefond L., Biochemistry 44:4805-4816(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 536-564 amino acids from the C-terminal region of human CARS2.
NCBI Gene ID #:
79587
NCBI Official Name:
Probable cysteine--tRNA ligase, mitochondrial
NCBI Official Symbol:
CARS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
Q9HA77
Protein GI Number:
74761587
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9HA77
User NOte:
Optimal dilutions for each application to be determined by the researcher.