PIGA Antibody
| Code | Size | Price |
|---|
| PSI-56-383-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, GlcNAc-PI synthesis protein, Phosphatidylinositol-glycan biosynthesis class A protein, PIG-A, PIGA
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a protein required for synthesis of
N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first
intermediate in the biosynthetic pathway of GPI anchor. The GPI
anchor is a glycolipid found on many blood cells and which serves
to anchor proteins to the cell surface. Paroxysmal nocturnal
hemoglobinuria, an acquired hematologic disorder, has been shown to
result from mutations in this gene. Alternate splice variants have
been characterized. A related pseudogene is located on chromosome
12.
Background References:
- Borowitz, M.J., et al. CytometryClin Cytom 78(4):211-230(2010)
- Peruzzi, B., et al. Mutat. Res. 705(1):3-10(2010)
- Araten, D.J., et al. Mutat. Res. 686 (1-2), 1-8 (2010) :
- Iida, Y., et al. Blood 83(11):3126-3131(1994)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PIGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-484 amino acids from the C-terminal region of human PIGA.
NCBI Gene ID #:
5277
NCBI Official Name:
Phosphatidylinositol N-acetylglucosaminyltransferase subunit A
NCBI Official Symbol:
PIGA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
P37287
Protein GI Number:
585696
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P37287
User NOte:
Optimal dilutions for each application to be determined by the researcher.