Dnmt3L Antibody
| Code | Size | Price |
|---|
| PSI-55-168-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA (cytosine-5)-methyltransferase 3-like, DNMT3L
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined.
Background References:
- Chedin, F., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16916-16921 (2002). Kierszenbaum, A.L., Mol. Reprod. Dev. 63(3):269-272 (2002). Deplus, R., et al., Nucleic Acids Res. 30(17):3831-3838 (2002). Hata, K., et al., Development 129(8):1983-1993 (2002). Burgers, W.A., et al., Trends Genet. 18(6):275-277 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Dnmt3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 357-388 amino acids from the C-terminal region of human Dnmt3L.
NCBI Gene ID #:
29947
NCBI Official Name:
DNA (cytosine-5)-methyltransferase 3-like
NCBI Official Symbol:
DNMT3L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
Q9UJW3
Protein GI Number:
334302913
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9UJW3
User NOte:
Optimal dilutions for each application to be determined by the researcher.