TAF8 Antibody

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ProSci
Product Code: PSI-57-029
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-029-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from Hela, U-251 MG cell line (from left to right), using TAF8 Antibody at 1:1000 at each lane.
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TAF8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysates from Hela, U-251 MG cell line (from left to right), using TAF8 Antibody at 1:1000 at each lane.
TAF8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Transcription initiation factor TFIID subunit 8, Protein taube nuss, TBP-associated factor 43 kDa, TBP-associated factor 8, Transcription initiation factor TFIID 43 kDa subunit, TAFII-43, TAFII43, hTAFII43, TAF8, TAFII43, TBN
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].
Background References:
  • Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009)
  • Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
  • Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
  • Soutoglou, E., et al. Mol. Cell. Biol. 25(10):4092-4104(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken
Immunogen:
This TAF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-178 amino acids from the Central region of human TAF8.
NCBI Gene ID #:
129685
NCBI Official Name:
Transcription initiation factor TFIID subunit 8
NCBI Official Symbol:
TAF8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
Q7Z7C8
Protein GI Number:
74723384
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q7Z7C8
User NOte:
Optimal dilutions for each application to be determined by the researcher.