TRUB1 Antibody

ProSci
Product Code: PSI-56-648
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-648-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in uterus tumor cell line lysates (35ug/lane).
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TRUB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in uterus tumor cell line lysates (35ug/lane).
TRUB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Probable tRNA pseudouridine synthase 1, 5499-, TRUB1, PUS4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]).
Background References:
  • Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
  • Deloukas, P., et al. Nature 429(6990):375-381(2004)
  • Zucchini, C., et al. Int. J. Mol. Med. 11(6):697-704(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TRUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the Central region of human TRUB1.
NCBI Gene ID #:
142940
NCBI Official Name:
Probable tRNA pseudouridine synthase 1
NCBI Official Symbol:
TRUB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
Q8WWH5
Protein GI Number:
74751577
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8WWH5
User NOte:
Optimal dilutions for each application to be determined by the researcher.