SMARCE1 Antibody

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ProSci
Product Code: PSI-57-670
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-670-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from SK-BR-3 cell line, using SMARCE1 Antibody at 1:1000 at each lane.
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SMARCE1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysate from SK-BR-3 cell line, using SMARCE1 Antibody at 1:1000 at each lane.
SMARCE1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, BAF57, SMARCE1, BAF57
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
Background References:
  • Hah, N., et al. Cancer Res. 70(11):4402-4411(2010)
  • Barrett, J.C., et al. Nat. Genet. 41(6):703-707(2009)
  • Pan, H., et al. Biochim. Biophys. Acta 1772(9):1075-1084(2007)
  • Camargo, L.M., et al. Mol. Psychiatry 12(1):74-86(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SMARCE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-335 amino acids from the C-terminal region of human SMARCE1.
NCBI Gene ID #:
6605
NCBI Official Name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
NCBI Official Symbol:
SMARCE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
Q969G3
Protein GI Number:
61247587
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q969G3
User NOte:
Optimal dilutions for each application to be determined by the researcher.