IMP4 Antibody

ProSci
Product Code: PSI-63-910
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-910-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
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IMP4 Antibody IHC analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
IMP4 Antibody IHC analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
U3 small nucleolar ribonucleoprotein protein IMP4, U3 snoRNP protein IMP4, Brix domain-containing protein 4, IMP4, BXDC4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
IMP4 forms a ternary complex with IMP3 (MIM 612980) and MPP10 (MPHOSPHO10; MIM 605503) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing.
Background References:
  • Hillier,L.W. Nature 434 (7034), 724-731 (2005)
  • Andersen,J.S.Nature 433 (7021), 77-83 (2005)
  • Granneman,S.Nucleic Acids Res. 31 (7), 1877-1887 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This IMP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-243 amino acids from the C-terminal region of human IMP4.
NCBI Gene ID #:
92856
NCBI Official Name:
U3 small nucleolar ribonucleoprotein protein IMP4
NCBI Official Symbol:
IMP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
Q96G21
Protein GI Number:
73621060
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96G21
User NOte:
Optimal dilutions for each application to be determined by the researcher.