RM50 Antibody
| Code | Size | Price |
|---|
| PSI-56-740-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
39S ribosomal protein L50, mitochondrial, L50mt, MRP-L50, MRPL50
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
Mammalian mitochondrial ribosomal proteins are encoded by
nuclear genes and help in protein synthesis within the
mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
small 28S subunit and a large 39S subunit. They have an estimated
75% protein to rRNA composition compared to prokaryotic ribosomes,
where this ratio is reversed. Another difference between mammalian
mitoribosomes and prokaryotic ribosomes is that the latter contain
a 5S rRNA. Among different species, the proteins comprising the
mitoribosome differ greatly in sequence, and sometimes in
biochemical properties, which prevents easy recognition by sequence
homology. This gene encodes a putative 39S subunit protein and
belongs to the L47P ribosomal protein family. Pseudogenes
corresponding to this gene are found on chromosomes 2p, 2q, 5p, and
10q.
Background References:
- Humphray, S.J., et al. Nature 429(6990):369-374(2004)
- Zhang, Z., et al. Genomics 81(5):468-480(2003)
- Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RM50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-147 amino acids from the C-terminal region of human RM50.
NCBI Gene ID #:
54534
NCBI Official Name:
39S ribosomal protein L50, mitochondrial
NCBI Official Symbol:
MRPL50
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
Q8N5N7
Protein GI Number:
118573685
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8N5N7
User NOte:
Optimal dilutions for each application to be determined by the researcher.