EPRS Antibody

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ProSci
Product Code: PSI-62-995
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-995-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase, Cell proliferation-inducing gene 32 protein, Glutamatyl-prolyl-tRNA synthetase, Glutamate--tRNA ligase, Glutamyl-tRNA synthetase, GluRS, Proline--tRNA ligase, Prolyl-tRNA synthetase, EPRS, GLNS, PARS, QARS, QPRS
Application Note:
For WB starting dilution is: 1:8000

For IHC-P starting dilution is: 1:10~50
Background:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. EPRS is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species.
Background References:
  • Jia,J., Mol. Cell 29 (6), 679-690 (2008)
  • Beausoleil,S.A., Nat. Biotechnol. 24 (10), 1285-1292 (2006)
  • Kato,T., Cancer Res. 65 (13), 5638-5646 (2005)
  • Sampath,P., Cell 119 (2), 195-208 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EPRS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-314 amino acids from the N-terminal region of human EPRS.
NCBI Gene ID #:
2058
NCBI Official Name:
Bifunctional glutamate/proline--tRNA ligase
NCBI Official Symbol:
EPRS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
171 kDa
Protein Accession #:
P07814
Protein GI Number:
288558855
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P07814
User NOte:
Optimal dilutions for each application to be determined by the researcher.