VARS Antibody

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ProSci
Product Code: PSI-63-235
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-235-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-VARS Antibody in K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the VARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-VARS Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the VARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Valine--tRNA ligase, Protein G7a, Valyl-tRNA synthetase, ValRS, VARS, G7A, VARS2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. This protein belongs to the class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.
Background References:
  • Jiang,S., FEBS Lett. 579 (27), 6049-6054 (2005)
  • Bonnefond,L., Biochemistry 44 (12), 4805-4816 (2005)
  • Xie,T., Genome Res. 13 (12), 2621-2636 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This VARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-279 amino acids from the N-terminal region of human VARS.
NCBI Gene ID #:
7407
NCBI Official Name:
Valine--tRNA ligase
NCBI Official Symbol:
VARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
140 kDa
Protein Accession #:
P26640
Protein GI Number:
12644177
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P26640
User NOte:
Optimal dilutions for each application to be determined by the researcher.