ZNF197 Antibody

ProSci
Product Code: PSI-55-247
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-247-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in U251 cell line lysates (35ug/lane).
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ZNF197 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in U251 cell line lysates (35ug/lane).
ZNF197 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Zinc finger protein 197, Zinc finger protein with KRAB and SCAN domains 9, ZnF20, pVHL-associated KRAB domain-containing protein, ZNF197, ZKSCAN9, ZNF166
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
ZNF197 product belongs to the zinc finger protein superfamily, members of which are regulatory proteins characterized by nucleic acid-binding zinc finger domains. The encoded protein contains 20 tandemly arrayed C2H2-type zinc fingers, a Kruppel-associated box (KRAB) domain, and a SCAN box. This transcript turns over rapidly and contains 3' UTR AUUUA motifs, which are often a hallmark of rapid turnover. It is overexpressed in some thyroid papillary carcinomas. ZNF197 is located in a cluster of zinc finger genes at 3p21. Two alternatively spliced transcripts encoding different isoforms have been described.
Background References:
  • Li, Z., et al. EMBO J. 22(8):1857-1867(2003)
  • Gonsky, R., et al. Nucleic Acids Res. 25(19):3823-3831(1997)
  • Calabro, V., et al. Hum. Genet. 95(1):18-21(1995)
  • Pengue, G., et al. Hum. Mol. Genet. 2(6):791-796(1993)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ZNF197 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the N-terminal region of human ZNF197.
NCBI Gene ID #:
10168
NCBI Official Name:
Zinc finger protein 197
NCBI Official Symbol:
ZNF197
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
119 kDa
Protein Accession #:
O14709
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O14709
User NOte:
Optimal dilutions for each application to be determined by the researcher.