LARS Antibody

ProSci
Product Code: PSI-62-892
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-892-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of anti-LARS Antibody in 293 cell line lysates (35ug/lane)
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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-LARS Antibody in 293 cell line lysates (35ug/lane)

Further Information

Additional Names:
Leucine--tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
LARS, a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. This enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain.
Background References:
  • Lue,S.W.; Biochemistry 46 (15), 4466-4472 (2007)
  • Ling,C., J. Biol. Chem. 280 (41), 34755-34763 (2005)
  • Giles,R.E., Somatic Cell Genet.(5), 667-687 (1980)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1118-1145 amino acids from the C-terminal region of human LARS.
NCBI Gene ID #:
51520
NCBI Official Name:
Leucine--tRNA ligase, cytoplasmic
NCBI Official Symbol:
LARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
134 kDa
Protein Accession #:
Q9P2J5
Protein GI Number:
48428689
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9P2J5
User NOte:
Optimal dilutions for each application to be determined by the researcher.