Parg Antibody

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ProSci
Product Code: PSI-62-792
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-792-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Parg antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Parg antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Poly(ADP-ribose) glycohydrolase, PARG
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products.
Background References:
  • Meyer,R.G., Exp. Cell Res. 313 (13), 2920-2936 (2007)
  • Fisher,A.E., Mol. Cell. Biol. 27 (15), 5597-5605 (2007)
  • Keil,C., J. Biol. Chem. 281 (45), 34394-34405 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This Parg antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-421 amino acids from the C-terminal region of human Parg.
NCBI Gene ID #:
8505
NCBI Official Name:
Poly(ADP-ribose) glycohydrolase
NCBI Official Symbol:
PARG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
111 kDa
Protein Accession #:
Q86W56
Protein GI Number:
56417893
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q86W56
User NOte:
Optimal dilutions for each application to be determined by the researcher.