PUS3 Antibody

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ProSci
Product Code: PSI-55-454
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-454-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
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PUS3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
PUS3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
tRNA pseudouridine(38/39) synthase, tRNA pseudouridine synthase 3, tRNA pseudouridylate synthase 3, tRNA-uridine isomerase 3, PUS3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs (By similarity).
Background References:
  • Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
  • Chen, J., et al. Biochemistry 39(41):12723-12730(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PUS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-64 amino acids from the N-terminal region of human PUS3.
NCBI Gene ID #:
83480
NCBI Official Name:
tRNA pseudouridine(38/39) synthase
NCBI Official Symbol:
PUS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
Q9BZE2
Protein GI Number:
126302589
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BZE2
User NOte:
Optimal dilutions for each application to be determined by the researcher.