RPA43 Antibody

ProSci
Product Code: PSI-61-860
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-860-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis of RPA43 Antibody in Jurkat,K562 cell line lysates (35ug/lane)
2 / 2
RPA43 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of RPA43 Antibody in Jurkat,K562 cell line lysates (35ug/lane)
RPA43 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
DNA-directed RNA polymerase I subunit RPA43, Twist neighbor protein, TWISTNB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
RPA43 is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.
Background References:
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Yuan, X., et al. EMBO Rep. 3(11):1082-1087(2002)
  • Kosan, C., et al. Cytogenet. Genome Res. 97 (3-4), 167-170 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RPA43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-326 amino acids from the C-terminal region of human RPA43.
NCBI Gene ID #:
221830
NCBI Official Name:
DNA-directed RNA polymerase I subunit RPA43
NCBI Official Symbol:
TWISTNB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
Q3B726
Protein GI Number:
121942339
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q3B726
User NOte:
Optimal dilutions for each application to be determined by the researcher.