MCM4 Antibody
| Code | Size | Price |
|---|
| PSI-57-795-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21, MCM4, CDC21
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is one of the highly
conserved mini-chromosome maintenance proteins (MCM) that are
essential for the initiation of eukaryotic genome replication. The
hexameric protein complex formed by MCM proteins is a key component
of the pre-replication complex (pre_RC) and may be involved in the
formation of replication forks and in the recruitment of other DNA
replication related proteins. The MCM complex consisting of this
protein and MCM2, 6 and 7 proteins possesses DNA helicase activity,
and may act as a DNA unwinding enzyme. The phosphorylation of this
protein by CDC2 kinase reduces the DNA helicase activity and
chromatin binding of the MCM complex. This gene is mapped to a
region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a
DNA-activated protein kinase involved in the repair of DNA
double-strand breaks. Alternatively spliced transcript variants
encoding the same protein have been reported.
Background References:
- Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
- Qian, Z., et al. PLoS Pathog.(3), E1000814 (2010) :
- Ladstein, R.G., et al. BMC Cancer 10, 140 (2010) :
- Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Mouse
Immunogen:
This MCM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the C-terminal region of human MCM4.
NCBI Gene ID #:
4173
NCBI Official Name:
DNA replication licensing factor MCM4
NCBI Official Symbol:
MCM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
P33991
Protein GI Number:
68571766
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P33991
User NOte:
Optimal dilutions for each application to be determined by the researcher.