DDX5 Antibody

ProSci
Product Code: PSI-62-930
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-930-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of DDX5 antibody in T47D cell line lysates (35ug/lane).
2 / 2
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of DDX5 antibody in T47D cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68, DDX5, G17P1, HELR, HLR1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
DDX5 is putative RNA helicases. The protein is implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen.
Background References:
  • Ong S.E., Mittler G.Nat. Methods 1:119-126(2004)
  • Daub H., Olsen J.V.Mol. Cell 31:438-448(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse
Immunogen:
This DDX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 491-518 amino acids from the C-terminal region of human DDX5.
NCBI Gene ID #:
1655
NCBI Official Name:
Probable ATP-dependent RNA helicase DDX5
NCBI Official Symbol:
DDX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
69 kDa
Protein Accession #:
P17844
Protein GI Number:
129383
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P17844
User NOte:
Optimal dilutions for each application to be determined by the researcher.