PAP Antibody
| Code | Size | Price |
|---|
| PSI-55-991-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Poly(A) polymerase alpha, PAP-alpha, Polynucleotide adenylyltransferase alpha, PAPOLA, PAP
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
Background References:
- Rapti, A., et al. Mol. Cell. Biochem. 340 (1-2), 91-96 (2010) :
- Nan, H., et al. J. Invest. Dermatol. 129(9):2250-2257(2009)
- Olsen, J.V., et al. Cell 127(3):635-648(2006)
- Dettwiler, S., et al. J. Biol. Chem. 279(34):35788-35797(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This PAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-435 amino acids from the Central region of human PAP.
NCBI Gene ID #:
10914
NCBI Official Name:
Poly(A) polymerase alpha
NCBI Official Symbol:
PAPOLA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
P51003
Protein GI Number:
59803092
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P51003
User NOte:
Optimal dilutions for each application to be determined by the researcher.