H2AFX Antibody
| Code | Size | Price |
|---|
| PSI-57-219-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
Histones are basic nuclear proteins that are responsible
for the nucleosome structure of the chromosomal fiber in
eukaryotes. Two molecules of each of the four core histones (H2A,
H2B, H3, and H4) form an octamer, around which approximately 146 bp
of DNA is wrapped in repeating units, called nucleosomes. The
linker histone, H1, interacts with linker DNA between nucleosomes
and functions in the compaction of chromatin into higher order
structures. This gene encodes a member of the histone H2A family,
and generates two transcripts through the use of the conserved
stem-loop termination motif, and the polyA addition motif.
Background References:
- Roch-Lefevre, S., et al. Radiat. Res. 174(2):185-194(2010)
- Schmid, T.E., et al. Int. J. Radiat. Biol. 86(8):682-691(2010)
- Jiang, X., et al. FEBS Lett. 584(13):2926-2930(2010)
- Vasireddy, R.S., et al. Br. J. Cancer 102(10):1511-1518(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This H2AFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human H2AFX.
NCBI Gene ID #:
3014
NCBI Official Name:
Histone H2AX
NCBI Official Symbol:
H2AFX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
15 kDa
Protein Accession #:
P16104
Protein GI Number:
121992
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P16104
User NOte:
Optimal dilutions for each application to be determined by the researcher.