EIF4E2 Antibody

ProSci
Product Code: PSI-60-608
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-608-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of EIF4E2 Antibody in NCI-H460 cell line lysates (35ug/lane)
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Western blot analysis of EIF4E2 using rabbit polyclonal EIF4E2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.
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Western blot analysis of EIF4E2 Antibody in NCI-H460 cell line lysates (35ug/lane)
Western blot analysis of EIF4E2 using rabbit polyclonal EIF4E2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.

Further Information

Additional Names:
Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3, EIF4E2, EIF4EL3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Background References:
  • Rom, E., et al., J. Biol. Chem. 273(21):13104-13109 (1998).
  • Mao, M., et al., Proc. Natl. Acad. Sci. U.S.A. 95(14):8175-8180 (1998).
  • Tee, A.R., et al., FEBS Lett. 564 (1-2), 58-62 (2004) (): ().
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EIF4E2 antibody is generated from rabbits immunized with human EIF4E2 recombinant protein.
NCBI Gene ID #:
9470
NCBI Official Name:
Eukaryotic translation initiation factor 4E type 2
NCBI Official Symbol:
EIF4E2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
O60573
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O60573
User NOte:
Optimal dilutions for each application to be determined by the researcher.